ASTM D4012-1981(2002) 水中微生物的三磷酸腺甙含量的试验方法
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【英文标准名称】:StandardTestMethodforAdenosineTriphosphate(ATP)ContentofMicroorganismsinWater
【原文标准名称】:水中微生物的三磷酸腺甙含量的试验方法
【标准号】:ASTMD4012-1981(2002)
【标准状态】:现行
【国别】:
【发布日期】:1981
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:D19.24
【标准类型】:(TestMethod)
【标准水平】:()
【中文主题词】:微生物;水;含量测定;试验
【英文主题词】:adenosinetriphosphate;ATP;microorganisms;water
【摘要】:Arapidandroutineprocedurefordeterminingbiomassofthelivingmicroorganismsincultures,waters,wastewaters,andinplanktonandperiphytonsamplestakenfromsurfacewatersisfrequentlyofvitalimportance.However,classicaltechniquessuchasdirectmicroscopecounts,turbidity,organicchemicalanalyses,celltagging,andplatecountsareexpensive,time-consuming,ortendtounderestimatetotalnumbers.Inaddition,someofthesemethodsdonotdistinguishbetweenlivingandnonlivingcells.TheATPfirefly(luciferin-luciferase)methodisarapid,sensitivedeterminationofviablemicrobialbiomass.ATPistheprimaryenergydonorforlifeprocesses,doesnotexistinassociationwithnonlivingdetritalmaterial,andtheamountofATPperunitofbiomass(expressedinweight)isrelativelyconstant.(ATPpercellvarieswithspeciesandphysiologicalstateoftheorganism.)Thistestmethodcanbeusedto:5.3.1Estimateviablemicrobialbiomassincultures,waters,andwastewaters.5.3.2Estimatetheamountoftotalviablebiomassinplanktonandperiphytonsamples.5.3.3EstimatethenumberofviablecellsinaunispeciescultureiftheATPcontent(oriftheaverageamountofATP)percellisknown.5.3.4Estimateanddifferentiatebetweenzooplanktonic,phytoplanktonic,bacterial,andfungalATPthroughsizefractionationofwater,andwastewatersamples.5.3.5Measurethemortalityrateofmicroorganismsintoxicitytestsinentrainmentstudies,andinothersituationswherepopulationsorassemblagesofmicroorganismsareplacedunderstress.1.1Thistestmethodcoversthemeasurementofadenosinetriphosphate(ATP)inmicroorganismsinconcentrationsnormallyfoundinlaboratorycultures,waters,wastewaters,andinplanktonandperiphytonsamplesfromwaters.1.2KnowledgeoftheconcentrationofATPcanberelatedtoviablebiomassormetabolicactivity,orbyutilizinganaverageconcentration(oramount)ofATPpercell,anestimatedcountofmicroorganismscanbeobtainedinthecaseofunispeciescultures.1.3Thistestmethodoffersahighdegreeofsensitivity,rapidity,accuracy,andreproducibility.However,extremecaremustbetakenateachstepintheanalysistoensuremeaningfulandreliableresults.1.4Theanalystshouldbeawarethattheprecisionstatementpertainsonlytodeterminationsinreagentwaterandnotnecessarilyinthematrixbeingtested.1.5Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:Z16
【国际标准分类号】:13_060_50(EXAMINATIONOFWATERFORCHEMICALSUBSTANCES)
【页数】:4P.;A4
【正文语种】:
【原文标准名称】:水中微生物的三磷酸腺甙含量的试验方法
【标准号】:ASTMD4012-1981(2002)
【标准状态】:现行
【国别】:
【发布日期】:1981
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:D19.24
【标准类型】:(TestMethod)
【标准水平】:()
【中文主题词】:微生物;水;含量测定;试验
【英文主题词】:adenosinetriphosphate;ATP;microorganisms;water
【摘要】:Arapidandroutineprocedurefordeterminingbiomassofthelivingmicroorganismsincultures,waters,wastewaters,andinplanktonandperiphytonsamplestakenfromsurfacewatersisfrequentlyofvitalimportance.However,classicaltechniquessuchasdirectmicroscopecounts,turbidity,organicchemicalanalyses,celltagging,andplatecountsareexpensive,time-consuming,ortendtounderestimatetotalnumbers.Inaddition,someofthesemethodsdonotdistinguishbetweenlivingandnonlivingcells.TheATPfirefly(luciferin-luciferase)methodisarapid,sensitivedeterminationofviablemicrobialbiomass.ATPistheprimaryenergydonorforlifeprocesses,doesnotexistinassociationwithnonlivingdetritalmaterial,andtheamountofATPperunitofbiomass(expressedinweight)isrelativelyconstant.(ATPpercellvarieswithspeciesandphysiologicalstateoftheorganism.)Thistestmethodcanbeusedto:5.3.1Estimateviablemicrobialbiomassincultures,waters,andwastewaters.5.3.2Estimatetheamountoftotalviablebiomassinplanktonandperiphytonsamples.5.3.3EstimatethenumberofviablecellsinaunispeciescultureiftheATPcontent(oriftheaverageamountofATP)percellisknown.5.3.4Estimateanddifferentiatebetweenzooplanktonic,phytoplanktonic,bacterial,andfungalATPthroughsizefractionationofwater,andwastewatersamples.5.3.5Measurethemortalityrateofmicroorganismsintoxicitytestsinentrainmentstudies,andinothersituationswherepopulationsorassemblagesofmicroorganismsareplacedunderstress.1.1Thistestmethodcoversthemeasurementofadenosinetriphosphate(ATP)inmicroorganismsinconcentrationsnormallyfoundinlaboratorycultures,waters,wastewaters,andinplanktonandperiphytonsamplesfromwaters.1.2KnowledgeoftheconcentrationofATPcanberelatedtoviablebiomassormetabolicactivity,orbyutilizinganaverageconcentration(oramount)ofATPpercell,anestimatedcountofmicroorganismscanbeobtainedinthecaseofunispeciescultures.1.3Thistestmethodoffersahighdegreeofsensitivity,rapidity,accuracy,andreproducibility.However,extremecaremustbetakenateachstepintheanalysistoensuremeaningfulandreliableresults.1.4Theanalystshouldbeawarethattheprecisionstatementpertainsonlytodeterminationsinreagentwaterandnotnecessarilyinthematrixbeingtested.1.5Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:Z16
【国际标准分类号】:13_060_50(EXAMINATIONOFWATERFORCHEMICALSUBSTANCES)
【页数】:4P.;A4
【正文语种】:
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点击此处下载